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regions included in a bicistronic vector enhances and stabilizes follistatin gene expressions in both transgeniccells and transgenic mice

Xiaoming HU,Jing GUO,Chunling BAI,Zhuying WEI,Li GAO,Tingmao HU,Shorgan BOU,Guangpeng LI

《农业科学与工程前沿（英文）》 2016年 第3卷 第1期   页码 87-96 doi: 10.15302/J-FASE-2016087

摘要： In the present study, follistatin ( ) gene expression vectors with either a bicistronic gene transfer cassette alone, or a bicistron gene cassette carrying a matrix attachment region (MAR) were constructed and transfected to bovine fetal fibroblasts. Evaluations of both the integration and expression of exogenous indicated that the pMAR-CAG-FST-IRES-AcGFP1-polyA-MAR (pMAR-FST) vector had higher capacity to form monoclonal transgenic cells than the vector without MAR, though transient transfection and integration efficiency were similar with either construct. Remarkably, protein expression in transgenic cells with the pMAR-FST vector was significantly higher than that from the bicistronic vector. Exogenous was expressed in all of the pMAR-FST transgenic mice at F , F and F . Total muscle growth in F mice was significantly greater than in wild-type mice, with larger muscles in fore and hind limbs of transgenic mice. pMAR-FST transgenic mice were also found with more evenly distributed muscle bundles and thinner spaces between sarcolemma, which suggests a correlation between transgene expression-associated muscle development and the trend of muscle growth. In conclusion, a pMAR-FST vector, which excluded the resistant genes and frame structure, enhances and stabilizes gene expressions in both transfected cells and transgenic mice.

关键词： safety of transgenic     bicistron gene transfer body     transgenic mice     muscle development    

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Effects of the

Yi FANG,Xiangwei FU,Junjie LI,Ming DU,Baoyu JIA,Jinlong ZHANG,Xiaosheng ZHANG,Shien ZHU

《农业科学与工程前沿（英文）》 2014年 第1卷 第4期   页码 314-320 doi: 10.15302/J-FASE-2014038

摘要： This study was conducted to systematically assess the reproductive performance of transgenic ewes. In the transgenic founders (F ) and their positive offspring (F ), hematological and reproductive parameters and the global DNA methylation level in oocytes at various stages were analyzed. The values of the physiological and biochemical parameters determined from the blood samples did not differ significantly between the transgenic and wild-type ewes. Moreover, the transgenic ewes showed reproductive traits similar to the wild-type ewes. These traits included characteristics of puberty, the estrus cycle, estrus duration, gestation, the pregnancy rate and the superovulation response. Additionally, no significant differences were found between transgenic and wild-type ewes in the DNA methylation level of the oocytes at various stages. In summary, the preliminary evidence presented in this paper demonstrates that the presence of the transgene did not affect the reproductive performance in sheep.

关键词： TLR4 transgenic ewe     safety assessment     reproductive trait     oocyte     DNA methylation    

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油菜转基因育种研究进展

官春云

《中国工程科学》 2002年 第4卷 第8期   页码 34-39

摘要：

简要介绍了国外油菜转基因育种情况，包括转除草剂抗性基因，病原菌抗性基因，耐重金属基因， 影响种子贮藏产物基因，影响繁殖特性基因，医药和工业产品基因，以及启动子调节基因等。较详细介绍了湖 南农业大学在Bt毒蛋白基因转化甘蓝型油菜育成抗虫新品系、基因工程雄性不育系、恢复系选育和杂种优势利 用，以及反义FAD2基因转化甘蓝型油菜双低品种提高油酸含量的研究结果；浙江省农科院利用反义PEP基因 转化甘蓝型油菜提高种子含油量的研究结果。

关键词： 油菜     转基因     育种    

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美国转基因大西洋鲑产业化对我国的启示

胡炜,朱作言

《中国工程科学》 2016年 第18卷 第3期   页码 105-109 doi: 10.15302/J-SSCAE-2016.03.018

摘要：

2015年11月19日，美国食品药品监督管理局（FDA）批准了转全鱼生长激素基因（gh）大西洋鲑为第一种可供食用的转基因动物产品，从而取得世界转基因动物育种研究与产业化的历史性突破。经过了长达20年的严格审核，转gh大西洋鲑才获准产业化，不仅充分说明了对包括转基因鱼在内的创新产业的管理决策尤其需要尊重科学规律和客观事实；而且表明基础研究只是产业创新的因素之一，转基因知识的科学普及与企业的积极参与是推动高新技术发展和创新产业形成不可或缺的重要条件；高屋建瓴地制定我国基因改良农业品种市场准入的条例和操作细则已经刻不容缓。

关键词： 转基因     大西洋鲑     产业化     启示    

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Construction of multiple shRNA vectors targeting PEDV and TGEV and production of transgenic SCNT porcine

Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG

《农业科学与工程前沿（英文）》 2019年 第6卷 第1期   页码 66-72 doi: 10.15302/J-FASE-2018229

摘要：

Porcine viral diarrhea is an acute and highly contagious enteric disease of pigs that causes huge economic losses worldwide. Porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) are the main pathogens responsible for piglet viral diarrhea. However, currently there is no specific drug available for the effective treatment of viral diarrhea. Therefore, it is necessary to seek an effective method to diminish PEDV and TGEV infection rates. RNA interference has been applied successfully to inhibit the virus replication. It provides a potential strategy for breeding resistant pigs. In this study, four promoters and four short hairpin RNA (shRNA) vectors with LoxP sites at each end of the selectable marker genes were constructed to target PEDV and TGEV. These vectors were then transfected into porcine fetal fibroblasts, G418 resistant transfectants were confirmed by PCR and transgenic SCNT porcine blastocysts were obtained. These results have paved the way for future production of marker-free transgenic resistant to PEDV and TEGV pigs by SCNT.

关键词： piglet diarrhea     RNAi     PEDV     TGEV     transgenic resistance breeding    

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Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

《农业科学与工程前沿（英文）》 2014年 第1卷 第3期   页码 179-184 doi: 10.15302/J-FASE-2014020

摘要： Butyrylcholinesterase (BCHE) is a natural bioscavenger that protects humans against organophosphate toxicity. Due to the limited yield of human BCHE (hBCHE) when purifying from human plasma, it is necessary to find an alternative method to produce this protein. One potential method is to produce transgenic livestock that make modified milk containing high concentration of hBCHE. In this study, we cloned the gene into a human lactoferrin (hLF) bacterial artificial chromosome (BAC) construct to make a hLF-hBCHE BAC construct. Subsequently, we injected the BAC construct into pronuclei of mouse fertilized embryos and generated transgenic mice. Expression analysis showed that recombinant hBCHE (rhBCHE) was expressed efficiently in the mammary gland of the transgenic mice and the concentration of rhBCHE in the milk of individual mice ranged from 76±12 to 159±28 mg·L . Protein function tests showed that rhBCHE has the same enzymatic activity as the native hBCHE. Our results pave the way for making transgenic livestock to produce large quantities of rhBCHE.

关键词： recombinant human butyrylcholinesterase (rhBCHE)     human lactoferrin bacterial artificial chromosome (hLF BAC)     transgenic mice     milk    

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conventional mature B cells and compromised specific antibody response in bovine immunoglobulin μ heavy-chain transgenic

Min ZHANG,Xueqian CHENG,Dan CHU,Jingwen LIANG,Yi SUN,Li MA,Beilei XU,Min ZHENG,Meili WANG,Liming REN,Xiaoxiang HU,Qingyong MENG,Ran ZHANG,Ying GUO,Yunping DAI,Robert AITKEN,Ning LI,Yaofeng ZHAO

《农业科学与工程前沿（英文）》 2014年 第1卷 第2期   页码 158-173 doi: 10.15302/J-FASE-2014015

摘要： In this study, we introduced the bovine immunoglobulin μ heavy-chain gene (the orphaned gene on BTA11) into mouse germline cells. Bovine IgM was highly expressed in selected transgenic lines, and it largely inhibited rearrangements of the endogenous immunoglobulin heavy chain (IgH) genes in these lines. The forced expression of bovine IgM resulted in reduced numbers of pro- and pre-B cells but increased the number of immature B cells in the transgenic mice. Bovine IgM-expressing B cells can migrate from the bone marrow to the spleen, but most of the cells are arrested at the T1 transitional B cell stage, leading to a significantly lower number of T2 transitional and mature B cells in the spleen. Although the serum concentrations of endogenous IgM and IgG in the transgenic mice were significantly decreased, the IgA levels were slightly increased compared to the WT mice. The bovine IgM level in the serum was only one-tenth to one-fifth of that of endogenous mouse IgM, suggesting that most of the serum immunoglobulin were contributed by endogenous IgH gene-expressing B cells. These transgenic mice also exhibited a lower frequency of unique complementarity determining region 3 (CDR3) sequences in their VH repertoire and Vκ repertoire but exhibited an increased frequency of unique CDR3 in their Vλ repertoire. Compared to the WT mice, the transgenic mice had a significantly higher percentage of mouse IgM-expressing B cells that expressed λ chains. Finally, we showed that the transgenic mice were deficient in a specific antibody response to antigen stimulation.

关键词： bovine Ig μ heavy-chain     transgenic mice     B cell development     allelic exclusion     immune response     Ig repertoire    

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Urban design based on public safety-Discussion on safety-based urban design

Kaizhen CAI, Jianguo WANG

《结构与土木工程前沿（英文）》 2009年 第3卷 第2期   页码 219-227 doi: 10.1007/s11709-009-0023-4

摘要： Because of public safety problems in construction of urban surroundings, this paper expounds the necessity of the study on public safety-based urban design in perspective of modern city. It brings forward the concept of safety-based urban design and attempts to explore the basic connotation and contents with framework for studies.

关键词： urban public safety     urban design     safety-based urban design    

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中国食品安全科技发展方向讨论

魏益民,吴永宁,周乃元,潘家荣

《中国工程科学》 2005年 第7卷 第11期   页码 1-4

摘要：

从发展国家食品安全战略，强化国家食品安全科技的角度，提出中国食品安全科技优先发展的四大方向，即风险分析是食品安全科技战略的首选领域；“从农田到餐桌”整体控制体系是食品安全全程控制技术和操作规范的理论基础，是实施食品安全管理的重要技术手段；检测监测技术是预警、预防、应急的技术依据，应集中力量开发一批内控和标准检验必须的快速检测技术和标准检测技术；以政府、生产者、消费者为责任人主体的管理理念是食品安全管理的基本原则，食品链上的所有责任人应在保障食品质量和安全的问题上主动承担责任。

关键词： 食品安全     科技     战略     发展方向    

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Probabilistic safety assessment of self-centering steel braced frame

Navid RAHGOZAR, Nima RAHGOZAR, Abdolreza S. MOGHADAM

《结构与土木工程前沿（英文）》 2018年 第12卷 第1期   页码 163-182 doi: 10.1007/s11709-017-0384-z

摘要： The main drawback of conventional braced frames is implicitly accepting structural damage under the design earthquake load, which leads to considerable economic losses. Controlled rocking self-centering system as a modern low-damage system is capable of minimizing the drawbacks of conventional braced frames. This paper quantifies main limit states and investigates the seismic performance of self-centering braced frame using a Probabilistic Safety Assessment procedure. Margin of safety, confidence level, and mean annual frequency of the self-centering archetypes for their main limit states, including PT yield, fuse fracture, and global collapse, are established and are compared with their acceptance criteria. Considering incorporating aleatory and epistemic uncertainties, the efficiency of the system is examined. Results of the investigation indicate that the design of low- and mid-rise self-centering archetypes could provide the adequate margin of safety against exceeding the undesirable limit-states.

关键词： self-centering steel braced frame     mean annual frequency     safety assessment     confidence level     margin of safety    

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安全科学若干基础问题研究

吕保和

《中国工程科学》 2006年 第8卷 第11期   页码 57-61

摘要：

分析了安全的科学含义，引入了"灾事"的概念，指出安全科学的研究对象就是灾事，并进一步探讨了灾事的类型以及各类灾事之间的关系。阐述了构建安全科学的必要性和可行性，并从一个新的角度分析了安全科学的内涵以及构建安全科学的基本思路，论述了安全科学技术体系的层次结构。

关键词： 安全     灾事     安全科学     学科建设    

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State of Practice of Construction Site Safety in the USA

Fei Dai,Yoojung Yoon,Hota V. GangaRao

《工程管理前沿（英文）》 2016年 第3卷 第3期   页码 275-282 doi: 10.15302/J-FEM-2016035

摘要： The construction industry has long been plagued with a high number of fatalities in the USA. Much safety improvements have been made after the USA Congress passed the Occupational Safety and Health Act in 1970. However, this industry still suffers from a disproportionately high rate of work-related fatalities in comparison to other industries. To provide a holistic view of current construction safety practices and corresponding performance, this paper reviews statistics of construction fatalities and their causes. It then presents efforts led to prevent work-related accidents and injuries by the federal safety agencies and the industry. Additionally, advantages and limitations of current practices in terms of construction safety have been discussed along with promotion of aspects in construction work environment.

关键词： workplace safety     construction industry     safety strategy     accident prevention     review work    

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Analyzing construction safety through time series methods

Houchen CAO, Yang Miang GOH

《工程管理前沿（英文）》 2019年 第6卷 第2期   页码 262-274 doi: 10.1007/s42524-019-0015-6

摘要： The construction industry produces a large amount of data on a daily basis. However, existing data sets have not been fully exploited in analyzing the safety factors of construction projects. Thus, this work describes how temporal analysis techniques can be applied to improve the safety management of construction data. Various time series (TS) methods were adopted for identifying the leading indicators or predictors of construction accidents. The data set used herein was obtained from a large construction company that is based in Singapore and contains safety inspection scores, accident cases, and project-related data collected from 2008 to 2015. Five projects with complete and sufficient data for temporal analysis were selected from the data set. The filtered data set contained 23 potential leading indicators (predictors or input variables) of accidents (output or dependent variable). TS analyses were used to identify suitable accident predictors for each of the five projects. Subsequently, the selected input variables were used to develop three different TS models for predicting accident occurrences, and the vector error correction model was found to be the best model. It had the lowest root mean squared error value for three of the five projects analyzed. This study provides insights into how construction companies can utilize TS data analysis to identify projects with high risk of accidents.

关键词： time series     temporal     construction safety     leading indicators     accident prevention     forecasting    

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Global challenges of food safety for China

Joseph J. JEN

《农业科学与工程前沿（英文）》 2018年 第5卷 第3期   页码 291-293 doi: 10.15302/J-FASE-2017167

摘要：

Ever since the Melamine event, China has faced challenges of food safety both domestically and globally. With the economic development, the Chinese consumers are demanding high quality, nutritious, and safe foods from the food industry but are not willing to pay higher prices. The Chinese food chain system of mostly small and medium enterprises presents challenges to the government to monitor and implement food safety laws and regulations. The academia and media are learning to take on their responsibilities with some success. Discussions and potential solutions of the global challenges of the five pillars for the food safety in China are presented here.

关键词： food safety     challenges     food industry     government     academia     media     consumer    

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A review of the safety and efficacy of current COVID-19 vaccines

《医学前沿（英文）》 2022年 第16卷 第1期   页码 39-55 doi: 10.1007/s11684-021-0893-y

摘要： Vaccination is the most effective and feasible way to contain the coronavirus disease 2019 (COVID-19) pandemic. The rapid development of effective COVID-19 vaccines is an extraordinary achievement. This study reviewed the efficacy/effectiveness, immunogenicity, and safety profile of the 12 most progressed COVID-19 vaccines and discussed the challenges and prospects of the vaccine-based approaches in a global crisis. Overall, most of the current vaccines have shown safety and efficacy/effectiveness during actual clinical trials or in the real-world studies, indicating a development of pandemic control. However, many challenges are faced by pandemic control in terms of maximizing the effect of vaccines, such as rapid vaccine coverage, strategies to address variants with immune escape capability, and surveillance of vaccine safety in the medium- and long-terms.

关键词： COVID-19     SARS-CoV-2     vaccine     safety     efficacy     effectiveness     immunogenicity    

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标题 作者 时间 类型 操作

regions included in a bicistronic vector enhances and stabilizes follistatin gene expressions in both transgeniccells and transgenic mice

Xiaoming HU,Jing GUO,Chunling BAI,Zhuying WEI,Li GAO,Tingmao HU,Shorgan BOU,Guangpeng LI

期刊论文

Effects of the

Yi FANG,Xiangwei FU,Junjie LI,Ming DU,Baoyu JIA,Jinlong ZHANG,Xiaosheng ZHANG,Shien ZHU

期刊论文

油菜转基因育种研究进展

官春云

期刊论文

美国转基因大西洋鲑产业化对我国的启示

胡炜,朱作言

期刊论文

Construction of multiple shRNA vectors targeting PEDV and TGEV and production of transgenic SCNT porcine

Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG

期刊论文

Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

期刊论文

conventional mature B cells and compromised specific antibody response in bovine immunoglobulin μ heavy-chain transgenic

Min ZHANG,Xueqian CHENG,Dan CHU,Jingwen LIANG,Yi SUN,Li MA,Beilei XU,Min ZHENG,Meili WANG,Liming REN,Xiaoxiang HU,Qingyong MENG,Ran ZHANG,Ying GUO,Yunping DAI,Robert AITKEN,Ning LI,Yaofeng ZHAO

期刊论文

Urban design based on public safety-Discussion on safety-based urban design

Kaizhen CAI, Jianguo WANG

期刊论文

中国食品安全科技发展方向讨论

魏益民,吴永宁,周乃元,潘家荣

期刊论文

Probabilistic safety assessment of self-centering steel braced frame

Navid RAHGOZAR, Nima RAHGOZAR, Abdolreza S. MOGHADAM

期刊论文

安全科学若干基础问题研究

吕保和

期刊论文

State of Practice of Construction Site Safety in the USA

Fei Dai,Yoojung Yoon,Hota V. GangaRao

期刊论文

Analyzing construction safety through time series methods

Houchen CAO, Yang Miang GOH

期刊论文

Global challenges of food safety for China

Joseph J. JEN

期刊论文

A review of the safety and efficacy of current COVID-19 vaccines

期刊论文